Isolation of Herbicide-Resistant 4-Hydroxyphenylpyruvate Dioxygenase from Cultured Coptis japonica Cells

Yuling LIANG; Hiromichi MINAMI; Fumihiko SATO

doi:10.1271/bbb.80466

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Isolation of Herbicide-Resistant 4-Hydroxyphenylpyruvate Dioxygenase from Cultured Coptis japonica Cells

Yuling LIANG, Hiromichi MINAMI, Fumihiko SATO

著者情報

キーワード: 4-hydroxyphenylpyruvate dioxygenase, Coptis japonica, destosyl pyrazolate, benzobicyclon hydrolysate, herbicide resistance

ジャーナルフリー早期公開

論文ID: 80466

DOI https://doi.org/10.1271/bbb.80466

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 11 p.3059

詳細

抄録

4-Hydroxyphenylpyruvate dioxygenase (HPPD) catalyzes the formation of homogentisate from 4-hydroxyphenylpyruvate and O₂. In plants, HPPD has been identified as a molecular target for herbicides. We report the isolation and characterization of a cDNA encoding a HPPD from cultured Coptis japonica cells. Recombinant CjHPPD showed significantly higher half-maximum inhibitory concentration (IC₅₀) values for the HPPD-inhibiting herbicide destosyl pyrazolate than other plant HPPDs.

責任著者(Corresponding author)

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