Crystallization and Structural Analysis of Cytochrome c6 from the Diatom Phaeodactylum tricornutum at 1.5 Å Resolution

Hideharu AKAZAKI; Fumihiro KAWAI; Masaki HOSOKAWA; Toshiyuki HAMA; Hirotaka CHIDA; Takako HIRANO; Boon-Keng LIM; Nobuo SAKURAI; Wataru HAKAMATA; Sam-Yong PARK; Toshiyuki NISHIO; Tadatake OKU

doi:10.1271/bbb.80472

This article has now been updated. Please use the final version.

Crystallization and Structural Analysis of Cytochrome c₆ from the Diatom Phaeodactylum tricornutum at 1.5 Å Resolution

Hideharu AKAZAKI, Fumihiro KAWAI, Masaki HOSOKAWA, Toshiyuki HAMA, Hirotaka CHIDA, Takako HIRANO, Boon-Keng LIM, Nobuo SAKURAI, Wataru HAKAMATA, Sam-Yong PARK, Toshiyuki NISHIO, Tadatake OKU

Author information

Hideharu AKAZAKI
Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University
Fumihiro KAWAI
Protein Design Laboratory, Graduate School of Integrated Science, Yokohama City University
Masaki HOSOKAWA
Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University
Toshiyuki HAMA
Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University
Hirotaka CHIDA
Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University
Takako HIRANO
Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University
Boon-Keng LIM
Kajima Corporation, Kajima Technical Research Institute, Environmental Engineering and Bioengineering Group-Hayama
Nobuo SAKURAI
Kajima Corporation, Kajima Technical Research Institute, Environmental Engineering and Bioengineering Group-Hayama
Wataru HAKAMATA
Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University
Sam-Yong PARK
Protein Design Laboratory, Graduate School of Integrated Science, Yokohama City University
Toshiyuki NISHIO
Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University
Tadatake OKU
Bio-Organic Chemistry Laboratory, Graduate School of Bioresource Sciences, Nihon University

Keywords: cytochrome c₆, crystal structure, diatom, physicochemical property, structural stability

JOURNAL FREE ACCESS Advance online publication

Article ID: 80472

DOI https://doi.org/10.1271/bbb.80472

The final version of this article is now available: Vol. 73 (2009) , No. 1 p.189

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Abstract

We determined for the first time the crystal structure of diatom cytochrome c₆ from Phaeodactylum tricornutum at 1.5 Å resolution. The overall structure of the protein was classified as a class I c-type cytochrome. The physicochemical properties of the protein were examined by denaturation with guanidine hydrochloride and urea, and compared with those of other algal cytochrome c₆.

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