Isolation of Human Renin Inhibitor from Soybean: Soyasaponin I Is the Novel Human Renin Inhibitor in Soybean

Saori TAKAHASHI; Kazuyuki HORI; Mamoru SHINBO; Kazuyuki HIWATASHI; Takeshi GOTOH; Seihan YAMADA

doi:10.1271/bbb.80495

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Isolation of Human Renin Inhibitor from Soybean: Soyasaponin I Is the Novel Human Renin Inhibitor in Soybean

Saori TAKAHASHI, Kazuyuki HORI, Mamoru SHINBO, Kazuyuki HIWATASHI, Takeshi GOTOH, Seihan YAMADA

著者情報

キーワード: renin, renin-angiotensin system (RAS), inhibitor, soybean, soyasaponin I

ジャーナルフリー早期公開

論文ID: 80495

DOI https://doi.org/10.1271/bbb.80495

この記事には本公開記事があります。

The final version of this article is now available: Vol. 72 (2008) , No. 12 p.3232

詳細

抄録

We found human renin inhibitory activity in soybean and isolated the active compound, soybean renin inhibitor (SRI). The physico-chemical data on the isolated SRI were identical with those of soyasaponin I. SRI showed significant inhibition against recombinant human renin, with an IC₅₀ value of 30 μg/ml. Kinetic studies with SRI indicated partial noncompetitive inhibition, with a K_i value of 37.5 μM. On the other hand, SRI weakly inhibited pepsin, papain, and bromeline activities, but did not inhibit other proteinases, such as trypsin, kallikrein, angiotensin converting enzyme, and aminopeptidase M. Moreover, a significant (p<0.05) decrease in the systolic blood pressure of spontaneously hypertensive rats was observed when partially purified SRI was orally administrated at 40 mg/kg/d for 7 weeks. This is the first demonstration of a renin inhibitor from soybean, soyasaponin I.

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