Identification and Characterization of Two Alternative σ Factors of RNA Polymerase in the Deep-Sea Piezophilic Bacterium Shewanella violacea, Strain DSS12

Hiroaki KAWANO; Hiroyuki TAKAHASHI; Fumiyoshi ABE; Chiaki KATO; Koki HORIKOSHI

doi:10.1271/bbb.80523

This article has now been updated. Please use the final version.

Identification and Characterization of Two Alternative σ Factors of RNA Polymerase in the Deep-Sea Piezophilic Bacterium Shewanella violacea, Strain DSS12

Hiroaki KAWANO, Hiroyuki TAKAHASHI, Fumiyoshi ABE, Chiaki KATO, Koki HORIKOSHI

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Keywords: Shewanella violacea DSS12, alternative σ factor, extracytoplasmic function, extracytoplasmic function (ECF) family, core binding analysis

JOURNAL FREE ACCESS Advance online publication

Article ID: 80523

DOI https://doi.org/10.1271/bbb.80523

The final version of this article is now available: Vol. 73 (2009) , No. 1 p.200

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Abstract

Two genes for alternative σ factors, σ^E2 and σ^E3, classified in the extracytoplasmic function σ family for RNA polymerases, were identified in the deep-sea piezophilic bacterium Shewanella violacea DSS12. Amino acid alignments revealed that the domains for transcriptional functions were comparatively conserved compared with Escherichia coli σ^E in both proteins. Core-binding analysis suggested that both proteins function as σ factors.

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