High-Performance Liquid Chromatographic Purification of Oligomeric Procyanidins, Trimers up to Nonamers, Derived from the Bark of Jatoba (Hymenaea courbaril)

Katsunori SASAKI; Yasuko MATSUKURA; Kumiko SHIJIMA; Mika MIYAKE; Daisuke FUJIWARA; Yutaka KONISHI

doi:10.1271/bbb.80747

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High-Performance Liquid Chromatographic Purification of Oligomeric Procyanidins, Trimers up to Nonamers, Derived from the Bark of Jatoba (Hymenaea courbaril)

Katsunori SASAKI, Yasuko MATSUKURA, Kumiko SHIJIMA, Mika MIYAKE, Daisuke FUJIWARA, Yutaka KONISHI

著者情報

キーワード: Jatoba, procyanidin, polyphenol, polyethylene glycol column, thiolysis

ジャーナルフリー早期公開

論文ID: 80747

DOI https://doi.org/10.1271/bbb.80747

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 6 p.1274

詳細

抄録

Procyanidin oligomers with different degrees of polymerization (up to nonamers) were efficiently purified from the bark of Jatoba (Hymenaea courbaril) by using a recently developed chromatographic separation method. Purification relied on a hydrogen bonding interaction between phenolic hydroxyl groups of the procyanidins and polyethylene glycol (PEG)-coated resin in a packed column. The individual procyanidins were identified by using electrospray ionization mass spectrometry (ESI-MS) and verified by a thiolytic degradation analysis. Our results demonstrate that Jatoba bark contained a large amount of procyanidins from monomer to nonamers or higher polymers composed of only B-type linked units (flavan-3-ol units linked through C-4 to C-8 (or C-6)) of epicatechin (EC) without gallate esters.

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