Stable Expression of the Chlorocatechol Dioxygenase Gene from Ralstonia eutropha NH9 in Hybrid Poplar Cells

Yasunori OHMIYA; Toshiro ONO; Toru TANIGUCHI; Naoei ITAHANA; Naoto OGAWA; Kiyotaka MIYASHITA; Kunio OHMIYA; Kazuo SAKKA; Tetsuya KIMURA

doi:10.1271/bbb.80848

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Stable Expression of the Chlorocatechol Dioxygenase Gene from Ralstonia eutropha NH9 in Hybrid Poplar Cells

Yasunori OHMIYA, Toshiro ONO, Toru TANIGUCHI, Naoei ITAHANA, Naoto OGAWA, Kiyotaka MIYASHITA, Kunio OHMIYA, Kazuo SAKKA, Tetsuya KIMURA

著者情報

キーワード: chlorocatechol, poplar, Ralstonia eutropha, transgenic

ジャーナルフリー早期公開

論文ID: 80848

DOI https://doi.org/10.1271/bbb.80848

この記事には本公開記事があります。

The final version of this article is now available: Vol. 73 (2009) , No. 6 p.1425

詳細

抄録

The cbnA gene encoding chlorocatechol dioxygenase from the soil bacterium Ralstonia eutropha NH9 under the control of a modified cauliflower mosaic virus 35S promoter was introduced into a hybrid poplar (Populus tremula × P. tremuloides). Integration of the cbnA gene in transgenic poplar was confirmed by PCR and genomic Southern blot analysis. Expression of the cbnA gene was analyzed by Western blot analysis. Transgenic poplar calli efficiently converted 3-chlorocatechol to 2-chloro-cis,cis-muconate.

責任著者(Corresponding author)

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