Identification of Proteins Interacting with Selenocysteine Lyase

Ryuta TOBE; Hisaaki MIHARA; Tatsuo KURIHARA; Nobuyoshi ESAKI

doi:10.1271/bbb.90065

This article has now been updated. Please use the final version.

Identification of Proteins Interacting with Selenocysteine Lyase

Ryuta TOBE, Hisaaki MIHARA, Tatsuo KURIHARA, Nobuyoshi ESAKI

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Keywords: selenocysteine lyase, protein-protein interaction, yeast two-hybrid system, major urinary protein, selenophosphate synthetase

JOURNAL FREE ACCESS Advance online publication

Article ID: 90065

DOI https://doi.org/10.1271/bbb.90065

The final version of this article is now available: Vol. 73 (2009) , No. 5 p.1230

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Abstract

Yeast two-hybrid screening of mouse cDNA libraries was performed to identify proteins interacting with selenocysteine lyase (SCL), which decomposes L-selenocysteine. Several proteins related to spermatogenesis, protein synthesis, and cell viability/apoptosis were identified as potential interactors. Major urinary proteins 1 and 2 interacted with SCL and inhibited its activity. Coimmunoprecipitation revealed interactions between SCL and each of two selenophosphate synthetase isozymes.

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