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Piezo-Adapted 3-Isopropylmalate Dehydrogenase of the Obligate Piezophile Shewanella benthica DB21MT-2 Isolated from the 11,000-m Depth of the Mariana Trench
Department of Chemistry, College of Humanities and Sciences, Nihon University Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology
Takako SATO
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology
Hideyuki TAMEGAI
Department of Chemistry, College of Humanities and Sciences, Nihon University
Chiaki KATO
Extremobiosphere Research Center, Japan Agency for Marine-Earth Science and Technology
Published: November 23, 2009Received: June 24, 2009Available on J-STAGE: -Accepted: August 19, 2009
Advance online publication: November 07, 2009
Revised: -
3-Isopropylmalate dehydrogenase (IPMDH)-encoding leuB genes were obtained from the obligate piezophile Shewanella benthica DB21MT-2 and non-piezophile Shewanella oneidensis MR-1. The genes were expressed in Escherichia coli and the proteins were purified using His-tag. The estimated kinetic parameters of these enzymes indicated that IPMDH of S. benthica DB21MT-2 is more tolerant of high pressure than that of S. oneidensis MR-1. Thus such an adaptation is one of the mechanisms bacteria utilize for survival at high pressures.
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