Purification and cDNA Cloning of LaIT2, a Novel Insecticidal Toxin from Venom of the Scorpion Liocheles australasiae

Nobuto MATSUSHITA; Masahiro MIYASHITA; Yayoi ICHIKI; Takehiko OGURA; Eiji SAKURADANI; Yoshiaki NAKAGAWA; Sakayu SHIMIZU; Hisashi MIYAGAWA

doi:10.1271/bbb.90509

This article has now been updated. Please use the final version.

Purification and cDNA Cloning of LaIT2, a Novel Insecticidal Toxin from Venom of the Scorpion Liocheles australasiae

Nobuto MATSUSHITA, Masahiro MIYASHITA, Yayoi ICHIKI, Takehiko OGURA, Eiji SAKURADANI, Yoshiaki NAKAGAWA, Sakayu SHIMIZU, Hisashi MIYAGAWA

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Keywords: scorpion toxin, cDNA cloning, insect toxicity

JOURNAL FREE ACCESS Advance online publication

Article ID: 90509

DOI https://doi.org/10.1271/bbb.90509

The final version of this article is now available: Vol. 73 (2009) , No. 12 p.2769

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Abstract

The novel insecticidal toxin, LaIT2, was isolated from venom of the scorpion, Liocheles australasiae. The amino acid sequence of LaIT2 was determined by an Edman degradation analysis and subsequent cDNA cloning. LaIT2 is composed of 59 amino acids with three disulfide bridges, and shares sequence similarity to the scorpion β-KTx peptides.

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