Characterization of the palH Gene from Aspergillus oryzae

Mitsuko DOHMOTO; Yayoi INOUE; Akiko KOBAYASHI; Shinichi OHASHI; Motoaki SANO

doi:10.1271/bbb.90575

This article has now been updated. Please use the final version.

Characterization of the palH Gene from Aspergillus oryzae

Mitsuko DOHMOTO, Yayoi INOUE, Akiko KOBAYASHI, Shinichi OHASHI, Motoaki SANO

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Keywords: Aspergillus oryzae, palH, pacC, alkaline protease (Alp), real-time polymerase chain reaction (PCR)

JOURNAL FREE ACCESS Advance online publication

Article ID: 90575

DOI https://doi.org/10.1271/bbb.90575

The final version of this article is now available: Vol. 74 (2010) , No. 1 p.188

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Abstract

We disrupted the palH gene, which is known to participate in the ambient pH signal transduction pathway, in Aspergillus oryzae. palH disruption caused significant decreases in pacC expression and alkaline protease activity. Hence we believe that palH plays a very important role in controlling the alkaline protease level in A. oryzae.

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