Minimum Length of Homology Arms Required for Effective Red/ET Recombination

Rika FUJIMOTO; Toshihiko OSAKABE; Masumichi SAITO; Nobuyuki KUROSAWA; Masaharu ISOBE

doi:10.1271/bbb.90584

This article has now been updated. Please use the final version.

Minimum Length of Homology Arms Required for Effective Red/ET Recombination

Rika FUJIMOTO, Toshihiko OSAKABE, Masumichi SAITO, Nobuyuki KUROSAWA, Masaharu ISOBE

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Keywords: Red/ET recombination, glutaredoxin5 (Glrx5), mitochondria, short homology arms

JOURNAL FREE ACCESS Advance online publication

Article ID: 90584

DOI https://doi.org/10.1271/bbb.90584

The final version of this article is now available: Vol. 73 (2009) , No. 12 p.2783

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Abstract

The original protocol of Red/ET recombination requires 50-bp sequence homology with target vector on both sides of the DNA fragment. To make it more cost effective, we investigated to determine the minimal length of homology required for the system to work. We found that a homology of 9-bp was sufficient to achieve homologous recombination with more than 50% efficiency.

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