Gene Cloning, Bacterial Expression, and Purification of a Novel Rice (Oryza sativa L.) Ubiquitin-Related Protein, RURM1

Takuji TSUKIYAMA; Jongwon LEE; Yutaka OKUMOTO; Masayoshi TERAISHI; Takatoshi TANISAKA; Kuniyo INOUYE

doi:10.1271/bbb.90692

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Gene Cloning, Bacterial Expression, and Purification of a Novel Rice (Oryza sativa L.) Ubiquitin-Related Protein, RURM1

Takuji TSUKIYAMA, Jongwon LEE, Yutaka OKUMOTO, Masayoshi TERAISHI, Takatoshi TANISAKA, Kuniyo INOUYE

著者情報

キーワード: Escherichia coli, glutathione S-transferase (GST)-fusion protein, Oryza sativa, recombinant protein, ubiquitin-like protein

ジャーナルフリー早期公開

論文ID: 90692

DOI https://doi.org/10.1271/bbb.90692

この記事には本公開記事があります。

The final version of this article is now available: Vol. 74 (2010) , No. 2 p.430

詳細

抄録

A rice ubiquitin-related modifier-1 (Rurm1) gene was cloned and transformed in Escherichia coli. We successfully expressed the RURM1 protein as a glutathione S-transferase (GST)-fusion protein by cultivating the E. coli cells at 16 °C for 16 h. After cleavage of GST, we obtained a single protein of 12 kDa. This protein was identified as the RURM1 protein by western blot analysis.

責任著者(Corresponding author)

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