Cell Culture and Motility Study on a Polymer Surface with a Nanometer-Scaled Stripe Structure

Yasuyuki SAKAMOTO; Kiichi SATO; Mitsuru ABO; Takehiko TSUKAHARA; Takehiko KITAMORI; Keiko ABE; Etsuro YOSHIMURA

doi:10.1271/bbb.90771

This article has now been updated. Please use the final version.

Cell Culture and Motility Study on a Polymer Surface with a Nanometer-Scaled Stripe Structure

Yasuyuki SAKAMOTO, Kiichi SATO, Mitsuru ABO, Takehiko TSUKAHARA, Takehiko KITAMORI, Keiko ABE, Etsuro YOSHIMURA

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Keywords: nanostructure, polydimethylsiloxane, cell morphology, cell migration, cell motility

JOURNAL FREE ACCESS Advance online publication
Supplementary material

Article ID: 90771

DOI https://doi.org/10.1271/bbb.90771

The final version of this article is now available: Vol. 74 (2010) , No. 3 p.569

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Abstract

We developed a large cell culture surface with a nanostripe structure by paving polydimethylsiloxane (PDMS) replicas of a glass mold. The stripe structure has a height of 180 nm and top width of 500 nm with 400-nm intervals between stripes. Human stomach cancer SH-10-TC cells cultured on the surface changed their morphology to elongated shapes parallel to the nanostripes. In addition, cell motility parallel to the stripes was greatly enhanced. These findings strongly suggest that the nanostripe structure affected the cell physiology.

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