Biochemical Studies on Vitamin B₁₂

Part X On Vitamin B₁₂-like Activity of Cobalt-Porphyrin-Derivative

抄録

(1) The alkali- and thermo-stablesubstance indicating vitamin B₁₂ -activity in plant kingdom was confirmed to be a porphyrin-derivative containing Cobalt by paper partition chromatography and bioautography.
(2) Culture experiments on Euglena and Lactobacillus leichmannii with Go-porphyrin-derivative prepared from radish-leaves were always successful by vitamin B₁₂-free basal medium when sup-plemented with 1, 2-diamino-4, 5-dime-thyl-benzene or 5, 6-dimethyl-benzimidazole.
(3) From these results, the physiologi-cal role of Co-porphyrin-derivative as one of the active components effecting on the intermediary motabolism of vitamin B₁₂ seems to be established.