Bulletin of the Agricultural Chemical Society of Japan
Online ISSN : 1881-1272
Print ISSN : 0375-8397
ISSN-L : 0375-8397
Studies on the Amylolytic System of the Black-koji Molds
Part I. On the Specificity of Saccharogenic Amylase Fraction
Seinosuke UEDA
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1956 Volume 20 Issue 3 Pages 148-154

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Abstract
Using preparations of dextrinogenic and saccharogenic amylase fractions of Black-koji amylase system, the conversion of starchess and glycogen was investigated.
1) The mode of conversion of amylous materials by dextrinogenic amylase fraction seems to be the same with that of malt α-amylase.
2) Saccharogenic amylase fraction hydro-lyzes the glutinous rice starch and glycogen completely to glucose in spite of the existence of 1, 6-linkage leaving no other sugar detectable by paper chromatography.
3) The adsorption treatment of this sac-charogenic amylase fraction with corn starch makes the hydrolysis of the β-LD from glycogen more difficult than that from potato starch just in opposition to the case with thee initial amylase fraction. It may, therefore, be considered here that some “debranching factor” exists in this amylase fraction.
4) Saccharogenic amylase fraction hydro lyzes potato starch to an extent of 90% leaving a sort of dextrin colored purple by iodine. Hence, it is suggested that potato starch has an anomalous linkage to the enzyme.
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