Separation and Identification of the Fatty Acids

Part XXI. Paper Chromatography of Fatty Acids as Their p-Bromophenacyl Ester Derivatives

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By reversed-phase paper chromatography, the simultaneous separations of all saturated and unsaturated fatty acids were successfully carried out on the same paper with the aid of p-bromophenacyl ester 2, 4-dinitrophenylhydrazones and their mercuric acetate addition compounds. When petroleum hydrocarbon (b. p. 140-170°) was used as the stationary solvent and methanol-acetic acid-petroleum hydrocarbon as the moving solvent, even saturated acids from C₄ to C₂₂, even monoethenoid acids from C₁₀ to C₂₂, and C₁₈ series from stearic to linolenic were well separated, respectively. Similar separations were also attained on paper impregnated with decalin and olive oil. From the results of several applications, this method has been found to be effective for the analysis of the component acids in natural fats.