Studies on the Proteolytic Enzymes of Black Aspergilli

Part I. Investigation of Strains Producing Superior Proteinase Yields in Black Aspergillus and the Crystallization of Proteolytic Enzyme from Aspergillus Saitoi

Abstract

A Procedure for the investigation of mold strains producing acid-stable proteinase is described. One hundred and eighty-nine strains of Black Aspergillus were tested. In the over-all pH range, the greatest activity was observed at pH 2.5. Individual mold strains varied in its ability to attack various substrates. Purification and crystallization procedures of the proteolytic enzyme from Aspergillus Saitoi, R-1149, are described.