Abstract
The phenolsulphatase of Aerobacter aerogenes was found to be formed in cell suspensions containing tyramine and casein hydrolyzate under aerobic conditions in a few hours.
Factors affecting phenolsulphatase synthesis in cell suspensions were studied.
The relationship between the rate of enzyme activity formed and the level of tyramine concentration in 10-4M to 10-3M is linear. The optimum concentration of tyramine for its utilization and phenolsulphatase induction is in both cases 5×10-3M. The opt. concentration of casamino acids for phenolsulphatase induction is 0.2 per cent and the opt. pH is 7.2. Agents such as 2, 4-dinitrophenol, sodium azide, arsenate and potassium cyanide inhibit the induction of this enzyme. The external inducer of phenolsulphatase should be highly specific. On addition of tyramine, hydroxytyramine and noradrenalin are effective, although the effect of noradrenalin is very weak.
