Abstract
By employing the paste-medium technique, the effects of some conditions of medium on the protease production by Asp. soyae KS were investigated. The medium of initial pH of 7-8, initial moisture content of 50 per cent, high C/N ratio, and of rice bran, copra meal had each favorable influence on protease production. Protease which was produced in the paste culture was observed in the mycelium as well as in the medium, while in the case of liquid culture it was mostly secreted into the medium. The secreted protease in the pastemedium was found to difuse to the depth of 2.5mm under the surface of the mycelium.
