Abstract
The following results were obtained from the fractionation of the saccharogenic amylase fraction:
(1) The debranching active principle precipitates at a lower concentration of ammonium sulfate than in the case of saccharogenic amylase.
(2) In paper electrophoresis, the debranchng active principle readily moves to anode.
(3) In fractionation with (NH4)2SO4, as well as in paper electrophoresis, debranching activity, free from saccharogenic activity could not be observed.
(4) From the results of electrophoresis, rivanol treatment and corn starch adsorption, it has been shown that the saccharogenic amylase fraction contains two types of saccharogenic amylases one possessing debranching activity and the other not.
