Studies on the Metabolism of D-Amino Acid in Microorganisms

Part V. Oxidation of D-Glutamic Acid by Cell-free Extracts of Aerobacter Strain A

Abstract

It is confirmed by a new method for the determination of D-glutamic acid, that Aerobacter strain A rapidly metabolizes _D-glutamic acid, while it only shows feeble metabolic activity towards L-glutamic acid when it is grown on a DL-glutamate-K₂HPO₄ medium. A specific D-glutamic oxidase is demonstrated in the cell-free extracts of Aerobacter strain A. This enzyme seems to be different from D-glutamic-aspartic oxidase obtained from Aspergillus ustus by the authors, since the former has no activity towards D-aspartic acid.