Studies on Phyto-peroxidase

Part XII. Amino Acid Composition of Japanese-radish Peroxidase a

抄録

The amino acid composition of hydrolysates of crystalline Japanese-radish peroxidase a has been determined mainly by chromatography on columns of Dowex 50-X8. The recoveries of the residues and the nitrogen were 93.52 and 100.90 per cent respectively, both including those of the carbohydrate residues and protohematin. The analyses indicate the presence of the following 384 amino acid residues, 65 carbohydrate residues and I protohematin in the molecule of peroxidase a (mol. wt. 54500): Asp₅₁Glu₂₆Gly₆Ala₆₄Val₂₀Leu₃₆Ileu₁₅Ser₅₁Thr₂₇(Cys-)₁₀Met₄Pro₁₇-Hypro₁₂Phe₁₈Tyr₃Try₂ His₄Lys₇Arg11(-CONH₂)₅₀Mannose₃₅Xylose₁₁Arabinose₄Hexosamine₁₅Protohe-matin₁.