Bulletin of the Agricultural Chemical Society of Japan
Online ISSN : 1881-1272
Print ISSN : 0375-8397
ISSN-L : 0375-8397
Action of Lysozyme and Some Microbial Enzymes on Acid-Resistant Organisms
Studies on Bacteriolytic Substances produced by Microorganisms, Part 5
Yukio SATOMURAShigetaka OKADAJuichiro FUKUMOTO
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1959 年 23 巻 3 号 p. 145-149

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(1) Non acid-fast cells of an avian tubercle bacillus were easily lysed by lysozyme, whereas acid-fast cells could be digested by a successive attack of lysozyme followed by Sclerotinia-enzyme. Bacterical lysozyme, trypsin and pancreatin, however, had no synergistic action with lysozyme. The synergistic factor contained in the Sclerotinia-enzyme extract was assumed to be a certain polysaccharidase.
2) The release of a mucopolysaccharide from the cell-wall during incubation with lysozyme increased by previously heating the cells at about 60° for 10min. The effect of heat-treat-ment upon the sensitivity of cells to lysozyme, which was accompanied by a remarkable Nakamura effect, was concluded to be due to the activation of some autolytic enzymes.
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