Abstract
1. The L-glutamic acid dehydrogenase reaction was successfully coupled with hydrogenase reaction utilizing gaseous hydrogen for the reduction of α-ketoglutaric acid.
The system consisted of hydrogen, hydrogenase, methylviologen, diaphorase, DPN, L-glutamic acid dehydrogenase, α-ketoglutaric acid and ammonium ion.
2. The amount of hydrogen uptake agreed well with the amount of α-ketoglutaric acid added.
The amount of L-glutamic acid produced was determined to be about 66 per cent of the hydrogen uptake.
3. The optimum pH for the reaction was found to be around pH 7.
