抄録
The effect of the amount of the amidol reagent on the rates of hydrolysis of some acid-labile phosphate compounds in the determination of inorganic orthophosphate according to the method of Allen2) and its modifications by Whelan and Bailey9) and by Nakamura12) is reported. It is shown in this paper that the most important factor in these methods is the temperature at which molybdenum blue color is developed. The rate constants of acid hydrolysis of adenosine triphosphate, glucose-l-phosphate, fructose-1, 6-diphosphate, phosphoryl (enol) pyruvate and inorganic pyrophosphate at various temperatures under the experimental conditions of Nakamura's method in the presence and absence of molybdate are also presented.
