抄録
1) Antiserum against the proteinase of Ps. myxogenes sp. was producted in rabbits by injection of the antigen proteinase.
2) By cross-absorption experiments, the crystalline proteinase used as the antigen was found to be of inhomogeneous nature even when it was recrystallized twice.
3) Inactivation of the enzyme by various treatments, such as heat, acid and EDTA, markably caused the same effect in decreasing its precipitin titer.
4) The proteolytic activity of the enzyme was found to be inhibited by its antibody.
5) The effect of the antiserum on various proteinases of other origins was studied. It was found that the antiserum inhibits the proteolytic activities of both strains of Ps. aeruginosa; while the proteinases of the other bacterial origins of different species and genus, and also those of mould and animal origins are never affected by the antiserum.
The author wishes to express his sincere thanks to Prof. H. Katagiri of Kyoto Univ. for his constant guidance and encouragement throughout this work. He is also indebted to Mr. S. Mayama of this Laboratory for his cooperation in carrying out this experiment. Thanks are also expressed to Dr. Nomoto of Scientific Research Institute, Dr. Yoshida of Noda Institute for Scientific Research, Nagase Ltd., Institute for Fermentation (Osaka) and Dept. of Science of Osaka Univ. for their kindly supplying various proteinase preparations and various different bacterial strains used here.
