Inhibition Pattern of Beet-Saponin on Amylose Formation by Potato Phosphorylase

Abstract

By using the multiple ascent technique, the authors have resolved the first several oligosaccharides of the product of initial stage of potato phosphorylase action in both the absence and the presence of beet-saponin with maltotriose as primer. The resolved chromatogram was sprayed with a mixture of G-l-P and phosphorylase, followed by spraying with iodine solution to locate the spots in which starch synthesis occurred.
Multi-chain mechanism of amylose formation from maltotriose and the suppression of length-ening of amylose chain by beet-saponin in the lag stage of enzyme action could be shown on paper chromatogram. No saccharides other than amylose series were recognized in the case of phosphorylase inhibition by beet-saponin.