抄録
A method for the preparatoin of diphosphopyridine nucleotide (DPN) from bakers' yeast is described. This method consists of partial purification of crude extract of yeast by charcoal chromatography according to Pontis and coworkers, ionexchange chromatography on Dowex-1 acetate, and precipitation of DPN as the free acid with ethanol. 0.7-1.1g of DPN with a purity of 85-90% was obtained from 5kg of fresh bakers' yeast by this method.
