抄録
In a previous paper, one of the authors (Innami) has reported the results of studies onthe isolation of mannase, “konjac” mannan-decomposing enzyme from the culture broth ofAerobacter mannanolyticus suzu-II, and if was confirmed that the enzyme preparation indicatedmarked activity for the decomposition of “konjac” mannan.
Afterwards, effects of various carbon sources on the growth response of the strain wasstudied, and it was found that the growth response of the bacteria may not be due to ordinaryTCA cycle members, whereas the strain did not respond to the media containing acetic acid, fumaric acid, succinic acid as a single carbon source. On the contrary, the bacteria increasedin the medium containing glyoxylate plus acetate as carbon sources. In conclusion, it wasclear that kornberg's glyoxylate-acetate cycle members formed by human digestive enzymesseem first to accelerate the growth response of the bacteria in the digestive tract, and successively, the mannase formed here to convert “konjac” mannan to D-glucose, D-mannose and others.
