Lipids of Algae

Part I. The Components of Unsaponifiable Matter of the Algae Scenedesmus

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A fresh cake of Scenedesmus cells was extracted with hot acetone, and the extracts were saponified. The yield of the unsaponifiable matter was 4.5% of the dry matter. Hydrocarbons and alcohols were extracted with petroleum ether from this unsaponifiable matter. The petroleum ether extracts were fractionated by the vacuum distillation. Each of the fractions was further separated by column chromatography or/and by the solubility difference of the urea adducts. Phytol was the major component of the unsaponifiable matter. Small amounts of n-hexadecane, n-hexacosene and n-eicosanol were isolated. On the other hand, the residue of the petroleum ether extraction was extracted with ether to separate sterols, of which the major component was found to be chondrillasterol.