Studies on Proteolytic Enzymes of Streptomyces griseus

Part I. Separation and Purification of Peptidase and Proteinase from Culture Media of Streptomyces griseus ATCC 3463

Abstract

Separation and purification of proteolytic enzymes of Streptomyces griseus ATCC 3463 were undertaken by fractional precipitation with ethanol and ammonium sulfate followed by dialysis and finally applying zone electrophoresis on starch. In the experiment, peptidase free from proteinase and the latter enzyme free from the former were obtained. The purity was increased approximately 90-fold with the peptidase and 8-fold with the proteinase.