Studies on Production of Nucleic Acid and its Related Compounds by Microorganisms

Part IV. Inosinic Acid Production by Organic and Biochemical Method

Abstract

Adenosine obtained from fermented broth was phosphorylated with fresh cells of Sacch. carlsbergensis IAM 4727 cultured in malt extract, to afford adenylic acid (Na-salt), which was then deaminated chemically to inosinic acid (Na-salt, IMP-Na). Thus, yield of IMP-Na obtained from malononitrile throughout the reactions was calculated, and as the result, it has been cleared that ca. 6g of IMP-Na was produced from 10g of malononitrile (yield ca. 10%). On the other hand, some reaction conditions of adenosine phosphorylation with yeast cells, i.e., concentration of adenosine and yeast cells in reaction mixture, substituting agent for toluene and direct phosphorylation of adenosine without isolation from fermented broth, were examined. Enzymic activities of various molds for deaminating AMP were also examined.