Turbidimetric Evaluation of Yeast Cell Amount

Abstract

It was found that the intercellular space of centrifuged sake yeast cultured in anaerobic medium is 0.263ml/g, which is larger than that of bakers' yeast. On the preparation of the calibration curve, this centrifuged sake yeast was used as standard substance.
The diameters of the majority of cells were included in a range of 3-8μ during the whole course of cultural development. A similar distribution was found on the cells cultured in an abnormal medium. From these results, clearly, the turbidimetric method for the evaluation of the cell amount of yeast cultured anaerobically is unaffected by the cultural conditions.