Abstract
(1) The authors determined the O2, uptake and CO2 evolution by the intact cells of Zygosaccharomyces soja (obtained by the cultivation in presence of 10-2M-monoiodoacetic acid and 10-3M-potassium cyanide) in a reaction mixture containing 6.6×10-2M-glucose, 10-2Mmonoiodoacetic acid and 10-3M-potassium cyanide, in the presence and absence of riboflavin. The R.Q. value in the presence of riboflavin was observed to be smaller than that in its absence.
(2) The same phenomena were observed when the intact cells of the same organism, obtained by the cultivation in absence of monoiodoacetic acid and cyanide, were used for the experiment.
(3) The authors found that cresyl violet, methylene blue, α-naphthoquinone and thionine also accelerated the O2 uptake of Z. soja in the aforementioned reaction mixture. This phenomenon was also observed when the cellfree extract was used for the experiment.
