Studies on the Metabolism of Azuki Seed Protein

Part V. Purification of Azuki Protease

Abstract

In order to investigate the role of a protease in protein metabolism during the germination of azuki seeds, the purification of the portease was carried out by employing fractionation with ammonium sulfate, dialysis against distilled water, and elution chromatography on a column of DEAE-cellulose. The enzyme preparation thus obtained was homogeneous in ultracentrifugal patterns.