Studies on the Accumulation of 5(4)-Amino-4(5)-imidazolecarboxamide byEscherichia coliin a Shaking Culture

Part III. The Effect of Addition of L-Tryptophan, and the Relationship between the Production of Indole and the Accumulation of 5(4)-Amino-4(5)-imidazolecarboxamide

抄録

In the previous paper the author reported that 5 (4)-amino-4 (5)-imidazolecarboxamide (AICA) was accumulated in peptone medium by Escherichia coli strain B grown as a shaking culture in the absence of sulfonamide inhibitor and glucose. It appeared from the further investigations that L-tryptophan would not replace peptone for the accumulation. However, it was found thatindole produced from L-tryptophan by E. coli gave pink color by the Bratton nad Marshall method. In order to eliminate the effect of indole, the procedure by petroleum-ether treatment was applied and it was ascertained that E. coli had an ability to accumulate AICA and AICA-riboside in the peptone or L-tryptophan medium without glucose and sulfonamide inhibitor. But the concentrations of AICA and AICA-riboside measured by theabove procedure were smaller than those determined by the Bratton and Marshall method which measured indole produced by the bacteria at the same time.