Studies on the Acid-protease ofPaecilomyces variotiBainier TPR-220

Part I. Crystallization of the Acid-protease of Paecilomyces varioti Bainier TPR-220

Abstract

A fungus, Paecilomyces variotiBainier TPR-220 was found to produce an acid-protease on wheat bran culture (Koji). The protease showed the highest activity at pHs between 2.5 and 3.0 on casein as substrate. The enzyme was stable at pHs from 2.5 to 6.0 at 40°C for forty minutes. By crystallization from 50% acetone solution the specific activity of the protease was increased five times as high as that of the original crude enzyme preparation.