Studies on Peptidases of Aspergillus oryzae

Part II. A Survey of Dipeptidase and Aminopolypeptidase Activities of Various Molds, and a Study of Mold Culture Method and Enzyme Extraction

Abstract

Screening experiments for dipeptidase and aminopolypeptidase from 40 strains of molds were conducted using Leu-Gly, Gly-Leu, Ala-Gly and Gly-Gly-Leu as substrates.
The strains of Aspergillus oryzae RO-0129 A-2, IAM-2600 and IAM-2616 showed strong activities of both dipeptidase and aminopolypeptidase.
Further, optimal condi tions for making culture as well as those for the extractions of the peptidases from the mycerial mats were investigated.