Abstract
In the previous paper, it was reported that the higher molecular weight luteose which consisted of β-1, 6-linked D-glucose units was made by Pen. aculeatuin var. apiculatum. The isolation of the microorganism which is able to produce β-D-1, 6-glucanase and some properties of this glucanase were described in this paper. The optimum reaction temperature for this enzyme was at about 40°C, and the optimum pH was at about 6.7. At the maximum degree of the hydrolysis, only D-glucose and gentiobiose were detected, and at this point, the hydrolysis degree of higher molecular weight luteose was about 55% as D-glucose. The formation of β-D-1, 6-glucanase was induced by the addition of higher molecular weight luteose. From the property of this enzyme, the authors proposed the name “lutease” for this enzyme.
