Sugar Isomerases

Part I. Production of D-Glucose Isomerase from Heterolactic Acid Bacteria

Abstract

Enzymatic activity for isomerizing D-glucose to D-fructose was demonstrated in the extracts from the D-xylose-grown cells of heterolactic acid bacteria; the enzyme was called the D-glucose isomerase. D-Xylose was found to be the only active source of carbon among the sugars tested for this enzyme production. Supplements of manganese and cobaltous ions to synthetic or malt extract media caused the remarkable acceleration on this enzyme production.