Abstract
Cytological effects of glycine on Bacillus subtilis var. conyloliquefaciens were compared between the cells of the glycine-sensitive parent and resistant mutant. Glycine induced disruption of the protoplasts which had been prepared by treating the glycine-sensitive cells with lysozyme. This effect of glycine was almost completely prevented by preincubating the protoplasts with spermine. The protoplasts prepared from the resistant cells were markedly stable in the presence of glycine. In this mutant, neither cell lysis nor cessation of the enzyme production by glycine occurred, contrary to the results obtained with the glycine-sensitive parent. Between both type of cells little difference could be observed in the metabolic activity for glycine, but free amino acid content was higher in the glycine-resistant cells than in the parent ones.
