Carbohydrates Oxidation by Pseudomonas albosesamae nov. sp.

Part II. Chemical Structure of the Product on Glucose and Gluconate Oxidation

Abstract

On shaking culture of the newly isolated bacterium, Pseutdomonas albosesamae, ⁷⁾ on glucose- or gluconate-containing medium, “a reducing acid” was produced in high yield. The product is produced specifically from glucose, gluconate and 2-ketogluconate, and not from other sugars. The product was isolated as a white to cream-colored, unstable, friable powder of calcium, barium or potassium satl by use of methanol precipitation technique.
On manometric studies of glucose, gluconate and 2-ketogluconate oxidation by lyophilized cells, 1.38, 0.99 and 0.48 equimolar consumption of oxygen were observed, respectively, and a common product was detected in the reaction mixture by paper chromatography. Periodate oxidation of “a reducing acid” gives the formation of oxalic and glycolic acid as oxidation products. Hardly any formaton of formaldehyde was observed. Ruff's oxidation of the reduced fermentation product, prepared by sodium borohydrate or catalytic reduction using platinum oxide as catalyst, gave the formation of D-arabinose and lesser amounts of L-xylose. On catalytic reduction by Raney nickel as the catalyst, 2-ketogluconate was formed as the main product. 2-ketogulonic acid was detected paper chromatographically, but the identification was unsuccessful.
The 2, 4-dinitrophenylhydrazone, analytically pure bis-2, 4-dinitrophenylhydrazone of diketogluconate, m. p. 156_??_157°C, [α]^24.5_D+57.2°, pyridine, 1 dm., was obtained as yellow needle crystals.
From the above results, “a reducing acid” was certified to be 2, 5-diketogluconic acid.