Bacterial Synthesis of Nucleotides

Part VI. Acceptor Specificity of Nucleoside Phosphotransferase (II): Phosphorylation of Deoxyribonucleosides

Abstract

1) Various kinds of deoxyribonucleosides were phosphorylated regardless of the replacement of acceptor ribonucleosides to deoxyribonucleosides.
2) The main product isomer was observed to be invariable without distinction of riboand deoxyribonucleosides, but the bacteria characterized to phosphorylate at C_5' of ribonucleoside produced 5'-isomer together with a little amount of 3'-isomer and “deoxyribo-nucleoside-X”, while the others characterized to synthesize 3'- and 2'-isomer phosphorylated only at C_3'.
3) The great difference present between the synthesis of ribonucleotides and deoxy ribonucleotides was on the synthesis of “de oxyribonucleoside-X”, probably deoxynucleo side-5', 3'-diphosphate, which was synthesizes from deoxyribonucleosides by only the bacteria of the former group.