Bacterial Synthesis of Nucleotides

Part VIII. Acceptor Specificity of Nucleoside Phosphotransferase (IV): Phosphorylation of Adenylic Acid

Abstract

1) Adenosine-5', 2'-diphosphate was found to be synthesized by incubating 2'-AMP with p-nitrophenylphosphate and a nucleoside phosphotransferase present in bacteria capable of phosphorylating at C_5', but was not synthesized from 3'-AMP.
2) Isomeric derivative, adenosine-5', 3' (or 2')-diphosphate, was also synthesized from 5'-AMP and the same phosphate donor by incubating with the nucleoside phosphotransferase present in bacteria which had been characterized to phosphorylate at C_3' or C_2'.
3) These findings strongly indicate that specific correlation was observed among the structure of acceptor, the nucleotide-isomer synthesized and the grouping of bacteria capable of phosphorylating nucleosides already proposed.