Studies on the Autolysis of Aspergillus oryzae

Part I. Conditions of Autolysis

抄録

Various conditions of the autolysis of Asp. oryzae were tested systematically and the fol-lowing results were obtained:
(1) Below 45°C, autolysis occurs faster at higher temperatures. It takes 1 day at 45°C and 3 days at 30°C for the autolysis to be completed.
(2) The optimum pH of autolysis is 5 as for the excretion of amino acids and nucleic acids.
(3) At the optimum conditions, 90_??_100% of nucleic acids, 75% of amino acids and 20% of sugars in the mycelia are excreted into medium.
(4) The autolysis occurs readily when the mycelia were incubated in water without glucose in anaerobic condition. And, the autolysis is inhibited by the addition of 2% of glucose under both aerobic and anaerobic conditions.
(5) The autolysis is stimulated by lipophilic compounds such as toluene and sodium salts of fatty acids. Hydrophilic groups have no stimulatory effect.