1965 Volume 29 Issue 7 Pages 665-671
To keep an enzyme stable in solution, the stabilities of the enzyme in various poly-hydric alcohols were investigated, and it was found that the enzyme in 70% aqueous sorbitol solution was strikingly stable against both storage at 33°C and heating at 80°C. It seems reasonable to ascribe this stability to such high osmotic pressure of the sorbitol concentration that decreased the viable counts of microorganisms. The reason why the sorbitol is the most effective among various polyhydric alcohols is now being investigated. This stable enzyme solution will be of good advantage as food improver, medicines or so on.
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