Four fractions of glucoamylase, different chromatographically from one another, were isolated and purified from a culture of Aspergillus oryzae on steamed rice. All these fractions were found to be homogeneous in sedimentation analysis, and had almost the same enzymatic activities in the hydrolysis of soluble starch. The limit of hydrolysis of starch was about 60%, which was very similar to those of Taka-amylase B of Okazaki and Taka-amylase B2 of Sawasaki.
