Abstract
Amino acid composition of crystalline Japanese-radish peroxidase c has been deter-mined by an automatic amino acid analyzer. Carbohydrate composition of this enzyme has also been investigated by means of the Tillmen and the Bial reactions. The analyses indicated that the peroxidase c had the following constituents in each molecule (mol. wt. 41, 500): Asp36Glu24Gly34Ala28Val27Leu27Ileu16Ser23Thr16(GYs-)10Met3Pro16Phe14Tyr2Try2His4 Lys8Arg30(-CONH2)27Hexose7Pentose2Hexosamine3Protohematin1. Since this composition was quite different from that of peroxidase a, two peroxidases could not be converted from each other, but they would be synthesized in vivo independently.
