Abstract
The enzyme system of NAD degradation was extracted from autolysate of Saccharomyces oviformis.
The enzymes were partially separated by ammonium sulfate fractionation and DEAE-cellulose column chromatography, and then the metabolic pathway of NAD in yeast was presented, in which four enzymes were contained. It has been found that, among them, the 5'nucleotidase has more affinity for AMP and the nucleosidase has strict affinity for nicotinamide riboside.
In the degradation of NAD in the yeast, nucleotide pyrophosphatase was main enzyme, but NADase, nucleotide pyrophosphorylase and adenosine deaminase seemed not to play an important role.
