The extracellular lipase from Candida paralipolytica required bile salts as the essential activator in the reaction mixture emulsified by polyvinyl alcohol. It has been found that some anionic surfactants can be used as the activator. The effect of bile salts and anionic surfactants on lipase activity has been studied in detail. Some cationic and non-ionic surfactants were not the activator but the inhibitor of the lipase.
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